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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
30/08/2022 |
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Actualizado : |
30/08/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CARRACELAS, B.; NAVAJAS, E.; VERA, B.; CIAPPESONI, G. |
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Afiliación : |
EMERITA BEATRIZ CARRACELAS MARQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BRENDA VERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Genome-wide association study of parasite resistance to gastrointestinal nematodes in Corriedale sheep. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Genes, 2022, vol. 13, issue 9, 1548. OPEN ACCESS. doi: https://doi.org/10.3390/genes13091548 |
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ISSN : |
e-ISSN 2073-4425 |
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DOI : |
10.3390/genes13091548 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 May 2022; Revised 19 August 2022; Accepted 23 August 2022; Published 27 August 2022.
Correspondence: gciappesoni@inia.org.uy -- Academic Editor: Qiuyue Liu.
License: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
This article belongs to the Collection Feature Papers in Animal Genetics and Genomics: https://www.mdpi.com/journal/genes/topical_collections/feature_paper_animal |
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Contenido : |
ABSTRACT.- Selection of genetically resistant animals is one alternative to reduce the negative impact of gastrointestinal nematodes (GIN) on sheep production. The aim of this study was to identify genomic regions associated with GIN resistance in Corriedale sheep by single-step genome-wide association studies (ssGWAS) using 170, 507 and 50K single nucleotide polymorphisms (SNPs). Analysis included 19,547 lambs with faecal egg counts (FEC) records, a pedigree file of 40,056 animals and 454, 711 and 383 genotypes from 170, 507 and 50K SNPs, respectively. Genomic estimated breeding values (GEBV) were obtained with single-step genomic BLUP methodology (ssGBLUP), using a univariate animal model, which included contemporary group, type of birth and age of dam as class fixed effects and age at FEC recording as covariate. The SNP effects as wells as p-values were estimated with POSTGSF90 program. Significance level was defined by a chromosome-wise False Discovery Rate of 5%. Significant genomic regions were identified in chromosomes 1, 3, 12 and 19 with the 170 SNP set, in chromosomes 7, 12 and 24 using the 507 SNP chip and only in chromosome 7 with the 50K SNP chip. Candidate genes located in these regions, using Oar_v4.0 as reference genome, were TIMP3, TLR5, LEPR and TLR9 (170 SNPs), SYNDIG1L and MGRN1 (507 SNP chip) and INO80, TLN2, TSHR and EIF2AK4 (50K SNP chip). These results validate genomic regions associated with FEC previously identified in Corriedale and other breeds and report new candidate regions for further investigation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- Selection of genetically resistant animals is one alternative to reduce the negative impact of gastrointestinal nematodes (GIN) on sheep production. The aim of this study was to identify genomic regions associated with GIN resistance in Corriedale sheep by single-step genome-wide association studies (ssGWAS) using 170, 507 and 50K single nucleotide polymorphisms (SNPs). Analysis included 19,547 lambs with faecal egg counts (FEC) records, a pedigree file of 40,056 animals and 454, 711 and 383 genotypes from 170, 507 and 50K SNPs, respectively. Genomic estimated breeding values (GEBV) were obtained with single-step genomic BLUP methodology (ssGBLUP), using a univariate animal model, which included contemporary group, type of birth and age of dam as class fixed effects and age at FEC recording as covariate. The SNP effects as wells as p-values were estimated with POSTGSF90 program. Significance level was defined by a chromosome-wise False Discovery Rate of 5%. Significant genomic regions were identified in chromosomes 1, 3, 12 and 19 with the 170 SNP set, in chromosomes 7, 12 and 24 using the 507 SNP chip and only in chromosome 7 with the 50K SNP chip. Candidate genes located in these regions, using Oar_v4.0 as reference genome, were TIMP3, TLR5, LEPR and TLR9 (170 SNPs), SYNDIG1L and MGRN1 (507 SNP chip) and INO80, TLN2, TSHR and EIF2AK4 (50K SNP chip). These results validate genomic regions associated with FEC previously identified in Corriedale and other breeds an... Presentar Todo |
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Palabras claves : |
FEC; Genomic regions; SsGWAS. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16636/1/genes-13-01548-v2.pdf
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Marc : |
LEADER 02850naa a2200229 a 4500
001 1063548
005 2022-08-30
008 2022 bl uuuu u00u1 u #d
022 $ae-ISSN 2073-4425
024 7 $a10.3390/genes13091548$2DOI
100 1 $aCARRACELAS, B.
245 $aGenome-wide association study of parasite resistance to gastrointestinal nematodes in Corriedale sheep.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 25 May 2022; Revised 19 August 2022; Accepted 23 August 2022; Published 27 August 2022. Correspondence: gciappesoni@inia.org.uy -- Academic Editor: Qiuyue Liu. License: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). This article belongs to the Collection Feature Papers in Animal Genetics and Genomics: https://www.mdpi.com/journal/genes/topical_collections/feature_paper_animal
520 $aABSTRACT.- Selection of genetically resistant animals is one alternative to reduce the negative impact of gastrointestinal nematodes (GIN) on sheep production. The aim of this study was to identify genomic regions associated with GIN resistance in Corriedale sheep by single-step genome-wide association studies (ssGWAS) using 170, 507 and 50K single nucleotide polymorphisms (SNPs). Analysis included 19,547 lambs with faecal egg counts (FEC) records, a pedigree file of 40,056 animals and 454, 711 and 383 genotypes from 170, 507 and 50K SNPs, respectively. Genomic estimated breeding values (GEBV) were obtained with single-step genomic BLUP methodology (ssGBLUP), using a univariate animal model, which included contemporary group, type of birth and age of dam as class fixed effects and age at FEC recording as covariate. The SNP effects as wells as p-values were estimated with POSTGSF90 program. Significance level was defined by a chromosome-wise False Discovery Rate of 5%. Significant genomic regions were identified in chromosomes 1, 3, 12 and 19 with the 170 SNP set, in chromosomes 7, 12 and 24 using the 507 SNP chip and only in chromosome 7 with the 50K SNP chip. Candidate genes located in these regions, using Oar_v4.0 as reference genome, were TIMP3, TLR5, LEPR and TLR9 (170 SNPs), SYNDIG1L and MGRN1 (507 SNP chip) and INO80, TLN2, TSHR and EIF2AK4 (50K SNP chip). These results validate genomic regions associated with FEC previously identified in Corriedale and other breeds and report new candidate regions for further investigation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
653 $aFEC
653 $aGenomic regions
653 $aSsGWAS
700 1 $aNAVAJAS, E.
700 1 $aVERA, B.
700 1 $aCIAPPESONI, G.
773 $tGenes, 2022, vol. 13, issue 9, 1548. OPEN ACCESS. doi: https://doi.org/10.3390/genes13091548
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
22/10/2021 |
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Actualizado : |
25/10/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
JOHNSON, S.N.; WATERMAN, J.M.; WUHRER, R.; ROWE, R.C.; HALL, C.R.; CIBILS-STEWART, X. |
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Afiliación : |
SCOTT N. JOHNSON; JAMIE M. WATERMAN; RICHARD WUHRER; RHIANNON C. ROWE; CASEY R. HALL; XIMENA CIBILS-STEWART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Hawkesbury Institute for the Environment, Western Sydney University, Penrith, New South Wales, Australia. |
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Título : |
Siliceous and non-nutritious: Nitrogen limitation increases anti-herbivore silicon defences in a model grass. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Journal of Ecology, 2021. Doi: https://doi.org/10.1111/1365-2745.13755 |
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DOI : |
10.1111/1365-2745.13755 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 29 July 2021/ Received: 24 March 2021/ Accepted: 28 June 2021. |
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Contenido : |
Abstract:
Silicon (Si) accumulation alleviates a diverse array of environmental stresses in many plants, including conferring physical resistance against insect herbivores. It has been hypothesised that grasses, in particular, utilise ?low metabolic cost? Si for structural and defensive roles under nutrient limitation. While carbon (C) concentrations often negatively correlate with Si concentrations, the relationship between nitrogen (N) status and Si is more variable. Moreover, the impacts of N limitation on constitutive physical Si defences (e.g. silica and prickle cells) against herbivores are unknown. We determined how N limitation affected Si deposition in the model grass Brachypodium distachyon and how changes in these constitutive defences impacted insect herbivore Helicoverpa armigera growth rates. We used scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry in conjunction with X-ray mapping (XRM) to quantify physical structures on leaves and determine Si deposition patterns. We also determined how N limitation and Si supply impacted the jasmonic acid (JA) pathway, the master regulator of induced defences against arthropod herbivores.N limitation reduced shoot growth by over 40%, but increased root mass (+21%), leaf Si concentrations (+50%) and the density of silica (+28%) and flattened prickle (+76%) cells. Energy-dispersive X-ray spectroscopy and XRM established that Si was being deposited in these structures, together with hooked prickle cells and macro-hairs. Herbivore relative growth rates (RGR) were more than 115% lower in Si-supplied plants compared to plants without Si supply and negatively correlated with leaf Si concentration and silica cell density. RGR was further reduced by N limitation and positively correlated with leaf N concentrations. Increases in JA concentrations following induction of the JA pathway were at least doubled by N limitation.Synthesis. Si accumulation and deposition were highly regulated by N availability, with N limitation promoting both constitutive Si physical defences and induction of the JA defensive pathway, in line with the resource availability hypothesis. These results indicate that grasses use ?low-cost Si? when resources are limited and suggest that plant productivity may benefit from optimising conventional fertilisers and Si fertilisation. MenosAbstract:
Silicon (Si) accumulation alleviates a diverse array of environmental stresses in many plants, including conferring physical resistance against insect herbivores. It has been hypothesised that grasses, in particular, utilise ?low metabolic cost? Si for structural and defensive roles under nutrient limitation. While carbon (C) concentrations often negatively correlate with Si concentrations, the relationship between nitrogen (N) status and Si is more variable. Moreover, the impacts of N limitation on constitutive physical Si defences (e.g. silica and prickle cells) against herbivores are unknown. We determined how N limitation affected Si deposition in the model grass Brachypodium distachyon and how changes in these constitutive defences impacted insect herbivore Helicoverpa armigera growth rates. We used scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry in conjunction with X-ray mapping (XRM) to quantify physical structures on leaves and determine Si deposition patterns. We also determined how N limitation and Si supply impacted the jasmonic acid (JA) pathway, the master regulator of induced defences against arthropod herbivores.N limitation reduced shoot growth by over 40%, but increased root mass (+21%), leaf Si concentrations (+50%) and the density of silica (+28%) and flattened prickle (+76%) cells. Energy-dispersive X-ray spectroscopy and XRM established that Si was being deposited in these structures, together with hooked prickle cel... Presentar Todo |
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Palabras claves : |
Insect herbivores; Jasmonate; Plant defence; Plant nutrients; Scanning electron microscopy; Silica; Silicon. |
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Thesagro : |
HIERBAS; INSECTOS; NUTRICION DE PLANTAS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03387naa a2200325 a 4500
001 1062494
005 2021-10-25
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1111/1365-2745.13755$2DOI
100 1 $aJOHNSON, S.N.
245 $aSiliceous and non-nutritious$bNitrogen limitation increases anti-herbivore silicon defences in a model grass.$h[electronic resource]
260 $c2021
500 $aArticle history: First published: 29 July 2021/ Received: 24 March 2021/ Accepted: 28 June 2021.
520 $aAbstract: Silicon (Si) accumulation alleviates a diverse array of environmental stresses in many plants, including conferring physical resistance against insect herbivores. It has been hypothesised that grasses, in particular, utilise ?low metabolic cost? Si for structural and defensive roles under nutrient limitation. While carbon (C) concentrations often negatively correlate with Si concentrations, the relationship between nitrogen (N) status and Si is more variable. Moreover, the impacts of N limitation on constitutive physical Si defences (e.g. silica and prickle cells) against herbivores are unknown. We determined how N limitation affected Si deposition in the model grass Brachypodium distachyon and how changes in these constitutive defences impacted insect herbivore Helicoverpa armigera growth rates. We used scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry in conjunction with X-ray mapping (XRM) to quantify physical structures on leaves and determine Si deposition patterns. We also determined how N limitation and Si supply impacted the jasmonic acid (JA) pathway, the master regulator of induced defences against arthropod herbivores.N limitation reduced shoot growth by over 40%, but increased root mass (+21%), leaf Si concentrations (+50%) and the density of silica (+28%) and flattened prickle (+76%) cells. Energy-dispersive X-ray spectroscopy and XRM established that Si was being deposited in these structures, together with hooked prickle cells and macro-hairs. Herbivore relative growth rates (RGR) were more than 115% lower in Si-supplied plants compared to plants without Si supply and negatively correlated with leaf Si concentration and silica cell density. RGR was further reduced by N limitation and positively correlated with leaf N concentrations. Increases in JA concentrations following induction of the JA pathway were at least doubled by N limitation.Synthesis. Si accumulation and deposition were highly regulated by N availability, with N limitation promoting both constitutive Si physical defences and induction of the JA defensive pathway, in line with the resource availability hypothesis. These results indicate that grasses use ?low-cost Si? when resources are limited and suggest that plant productivity may benefit from optimising conventional fertilisers and Si fertilisation.
650 $aHIERBAS
650 $aINSECTOS
650 $aNUTRICION DE PLANTAS
653 $aInsect herbivores
653 $aJasmonate
653 $aPlant defence
653 $aPlant nutrients
653 $aScanning electron microscopy
653 $aSilica
653 $aSilicon
700 1 $aWATERMAN, J.M.
700 1 $aWUHRER, R.
700 1 $aROWE, R.C.
700 1 $aHALL, C.R.
700 1 $aCIBILS-STEWART, X.
773 $tJournal of Ecology, 2021. Doi: https://doi.org/10.1111/1365-2745.13755
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